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郑从义, 吴海祥, 张楚瑜*, 王家富, 潘兹书, 李蕾, 曹晟, 伊光辉
科学通报,2004,48(8):822~826,-0001,():
-1年11月30日
猪瘟病毒在离体细胞培养条件下不引起宿主细胞病变,病毒与宿主细胞之间相互作用的研究一直没有合适的模型以猪瘟病毒中国标准强毒石门株为材料,通过基因工程的手段,在构建全基因组感染性克隆的基础上,对全基因组进行部分缺失,获得了7.5kh的亚基因组.以携带野生型猪瘟病毒的PK-15细胞为宿主,用亚基因组进行转染,获得到了能够诱导PK-15细胞产生CPE的亚基因组病毒,检测显示产生CPE的细胞培养物中野生型基因组和亚基因组共同存在猪瘟病毒致细胞病变模型的构建,为进一步研究其致病的分子机制开辟了新的方向。
猪瘟病毒, 基因组感染性克隆, 亚基因组, 干扰缺损颗粒, 致细胞病变效应
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【期刊论文】丙肝病毒全基因组cDNA克隆侵染细胞培养体系的建立
郑从义, 姚相杰①, 郭佳①, 郑从义①*, 方呈祥①, 屈三甫①, 陈震球②, 李中红②, 李信墙②*, 李卫云②
科学通报,2004,49(10):965~970,-0001,():
-1年11月30日
以含有丙肝病毒全基因组 cDNA的重组质粒(pHCV)为材料, 通过基因转染、重组痘病毒辅助使之在HeLa细胞中表达HCV病毒体,建立了一种新的HCV体外细胞培养系统。采用RT-PCR,荧光定量RT-PCR,链特异性RT-PCR,免疫印迹,免疫电子显微镜和电子显微镜负染技术跟踪检测HCV的增殖效率,结果显示,该培养体系中有HCV正链RNA的合成和HCV结构蛋白、非结构蛋白的表达,并组装成直径约47nm的HCV病毒体,病毒体可被偶联有胶体金的抗HCV结构蛋白E2的单抗标记;该体系产生的HCV病毒体滴度达107基因拷贝/mL,远远高于病人血清中的HCV基因拷贝数,也高于迄今报道的任何一种HCV细胞培养体系的病毒滴度,检测结果证明,转染细胞裂解上清中的HCV病毒体可以感染肝癌细胞系Huh7,并在感染细胞中增殖和合成负链RNA中间体,病毒滴度达106基因拷贝/mL。
丙型肝炎病毒(, HCV), , 全基因组, 侵染性cDNA, 克隆, 重组痘病毒, 细胞培养体系
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郑从义, Guo-Ping Yan, Ph.D, Professor* Cong-Yi Zheng, Professory Wei Cao, M.D.z Wei Li, M.S.* Li-Yun Li, Professor
Radiography (2003) 9, 35-41,-0001,():
-1年11月30日
Four DTPA and DOTA derivative ligands containing sulfonamide groups and their gadolinium complexes were prepared and characterized. Relaxivity studies showed that these gadolinium complexes possessed higher relaxation effectiveness than those of the corresponding ionic gadolinium complex Gd-DTPA and Gd-DOTA, respectively. In vitro cytotoxicity assay demonstrated that these gadolinium complexes have low cytotoxicity to the human liver cells (L-02). Magnetic resonance imaging (MRI) and experimental data of biodistribution in mice indicated that these gadolinium complexes containing sulfonamide groups could be used as the potential MRI contrast agents for Hepatoma (H22) and Ehrlich ascites carcinoma (EAC) in mice and specific organs such as the liver and could be mostly excreted by the kidneys.
magnetic resonance imaging (, MRI), , gadolinium complexes, sulfanilamide, sulfadiazine, relaxivity, target-specific.,
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郑从义, Liu Yuwena, Zhang Henga, Wang Cunxin a, Li Jiea, Li Huitin a, Li Zhaoyang b, Wu Jibin b, Zheng Congyi b, ∗
Thermochimica Acta 407(2003)113-116,-0001,():
-1年11月30日
In this study, microcalorimetric measurements were carried out on PK-15 cell line persistently infected by cholera swine fever virus (CSFV) and BHK-21 cell line infected by foot and mouth disease virus (FMDV). The aim of this investigation was to investigate the difference of energy metabolism between the different kinds of virus infections-persistent infection and cytocidal infection. The thermogenesis curves determined by using a LKB 2277 Bioactivity Monitor were significantly different. From these thermogenic curves, the maximum heat production rate Pm and total heat output Q are obtained. The results show that energy metabolism is different between persistent infection and cytocidal infection.
Virus, Microcalorimetry, Metabolism, Persistent infection, Cytocidal infection
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郑从义, Feng Liang, a Ping Wang, a Xiang Zhou, a Tao Li, b Zhaoyang Li, c Huakuan Lin, d, Dongzhao Gao, d Congyi Zhengc and Chengtai Wua, *
Bioorganic & Medicinal Chemistry Letters 14(2004)1901-1904,-0001,():
-1年11月30日
The stability constants for the formation of nickle(II) and cobalt(II) complexes of the ligand [1,4,7]triazecan-9-ol (L) were presented. Antitumor activity of two complexes was reported. Nuclei of [NiL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to cell cycle, and optimal induction of apoptosis was found by Flow-Cytometric analysis. But CoL complex did not exhibit introduction effects to BEL-7402 cells apoptosis; and could not perturb cell cycle. NiL and CuL complexes could cleave supercoiled DNA (pBR 322 DNA) to nicked and linear DNA, and DNA of cells treated with NiL or CuL complex was obviously damaged; while CoL complex only could cleave supercoiled DNA (pBR 322 DNA) to nicked DNA, and DNA of cells treated with CoL complex had no significant difference with control.
Macrocyclic polyamines, Metal complexes, Antitumor activity, DNA cleavage.,
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