Four DTPA and DOTA derivative ligands containing sulfonamide groups and their gadolinium complexes were prepared and characterized. Relaxivity studies showed that these gadolinium complexes possessed higher relaxation effectiveness than those of the corresponding ionic gadolinium complex Gd-DTPA and Gd-DOTA, respectively. In vitro cytotoxicity assay demonstrated that these gadolinium complexes have low cytotoxicity to the human liver cells (L-02). Magnetic resonance imaging (MRI) and experimental data of biodistribution in mice indicated that these gadolinium complexes containing sulfonamide groups could be used as the potential MRI contrast agents for Hepatoma (H22) and Ehrlich ascites carcinoma (EAC) in mice and specific organs such as the liver and could be mostly excreted by the kidneys.

In this study, microcalorimetric measurements were carried out on PK-15 cell line persistently infected by cholera swine fever virus (CSFV) and BHK-21 cell line infected by foot and mouth disease virus (FMDV). The aim of this investigation was to investigate the difference of energy metabolism between the different kinds of virus infections-persistent infection and cytocidal infection. The thermogenesis curves determined by using a LKB 2277 Bioactivity Monitor were significantly different. From these thermogenic curves, the maximum heat production rate Pm and total heat output Q are obtained. The results show that energy metabolism is different between persistent infection and cytocidal infection.

以含有丙肝病毒全基因组 cDNA的重组质粒（pHCV）为材料, 通过基因转染、重组痘病毒辅助使之在HeLa细胞中表达HCV病毒体，建立了一种新的HCV体外细胞培养系统。采用RT-PCR，荧光定量RT-PCR，链特异性RT-PCR，免疫印迹，免疫电子显微镜和电子显微镜负染技术跟踪检测HCV的增殖效率，结果显示，该培养体系中有HCV正链RNA的合成和HCV结构蛋白、非结构蛋白的表达，并组装成直径约47nm的HCV病毒体，病毒体可被偶联有胶体金的抗HCV结构蛋白E2的单抗标记；该体系产生的HCV病毒体滴度达107基因拷贝/mL，远远高于病人血清中的HCV基因拷贝数，也高于迄今报道的任何一种HCV细胞培养体系的病毒滴度，检测结果证明，转染细胞裂解上清中的HCV病毒体可以感染肝癌细胞系Huh7，并在感染细胞中增殖和合成负链RNA中间体，病毒滴度达106基因拷贝/mL。

The protonation constants and the stability constants for the formation of copper (II) complex of the ligand [1,4,7] Triazecan-9-ol (L) were presented. Antitumor activity of CuL complex was reported. Preliminary pharmacological tests showed that it had antitumor activity against HXO-RB44 and BEL-7402 cell lines in vitro. Nuclei of [CuL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to BEL-7402 cell lines cycle and further studies showed that it could cleave supercoiled DNA (pBR 322) to nicked and linear DNA.

Reactive oxygen species play an important role in the mediation of cell killing. But the mechanistic links between reactive oxygen species (ROS) and cell death remains unclear. There was a speculation that ROS, especially hydroxyl radicals can induce necrosis but not apoptosis in cells treated with copper-1,10-phenanthroline, IICu(OP)2. In this paper, liver carcinoma cell line (Bel-7402) was treated with IICu(OP)2 and its effect was examined by several means. Cells were found to undergo changes characteristic of apoptosis. Hoechst staining showed apoptotic body appeared in the cells induced by IICu(OP)2. When DNA extracted from the cells treated with IICu(OP)2 was analyzed by agarose gel electrophoresis it generated 'ladder' pattern of discontinuousDNAfragments. Sub-G1 peakwas detected in treated cells. Furthermore, two different flowcytometric methods were used, each allowing us to relate the apoptotic cells to the position the cell-cycle position. Apoptosis induced by IICu(OP)2 was limited to G1-phase cells. Using cyclin analysis, the expression of cyclin E in G1 was blocked. Thus, it was concluded that IICu(OP)2 can induceG1-phase specific apoptosis in Bel-7402.