用石蜡连续切片苏木精染色法，通过定量分析研究来伤和切断视神经后，斑马鱼视网膜神经节细胞、视杆和视连细胞密度的变化。结果发现，在损伤视神7-21d后，上述3种细胞的细胞核密度均呈减少趋势，节细胞减少的比率大于感光细胞，而感光细胞中视维细胞所受影响比视杆细胞更为明显；在夹伤和切断视神经两种情况中，后者引起民视网膜神经节细胞核密度的减少更为显著。上述结果表明，损伤视神经不但影响与其相逢的神经节细胞而且可逆向跨神经元地影响感光细胞的变化。由上述结果推测，由子损伤视神经使视网膜神经节细胞失去靶组织而引起的中各神经细胞密度减少是视网膜中神经细胞凋亡的表现。

花背蟾蜍蝌蚪角膜诱导过程中的组织化学变化，主要是在代谢活跃的表皮移植片蠕胞的细胞质中蛋白质核酸，特别是核糖核酸（RNA）的含量发生明显的变化，不同个体几科同时出现两个高峰，第一个高峰出现在移植手术后的初期，即从手术后到移植片愈合展平，它反映接受手术的蝌蚪可能借助代谢作用物南（蛋白质）的合成来修复损伤并使移植片与受体皮肤相愈合，第二个高峰出现的时间与移植片中色素消失开始的时间基本一致，初步断定此时细胞已开始向角膜分化，它可能与移植片透明和组织重建时新蛋白质的合成有关。

Previous studies had showen that when the paranecrotic skin of the tadpole was transplauted to the position of the excised cornea of a tadpole at the same stage, this piece of skin was transformed into a new cornea much more rapidly than the intake one. In this paper the authors made an attermpt to compare the ultrastructures of normal tadpole skin with that of paranecrotic skin caused by low con-centration of acetic acid (0.01N) and of ammonia water (0.001N). Certain ultrasturctural changes in the skin under the conditions of paranecrosis are found as follows: (1) The tonofilaments in the equder-mal cells become more or less agger-gasted and appeared loosened. These changes are more obvious in the skin of weak acid treatment. (2) The organelles in epidermal cells are decreased in quantity, numerous vacuoles are observed in the sytoplasm; the intercellular observed in the cytoplasm; the intercellular spaces between adja-cent cells have become narrowed than the normal. (3) The chromatin granules are found in condensed clumps scattered unevenly throughout the nucleus, lea-ving irregular spaces in the nucleus. (4) The dark and light periodic bands of the collagenous fibres in the basement membrane are obscure in contours. The changes mentioned above are discussed I nrelation to the possible mechanisms of quickening the process of corneal induction under the con-ditions of skin paranecrosis.

作用用光施和电镇研究了花背蟾蜍蝌蚪变态期角膜的发展。在后皮发育晚期，内外角膜在中央部位首先集合，在完全变态期角膜完全愈合，此对角膜上皮细胞增殖，上皮基质变为Baweman's膜，内外角膜之间的成纤维细胞和由它分泌的胶原纤维形成角膜基质，内角膜细胞形成单层的角膜内皮，它与角膜基质间Desccmers膜最快形成。

电子显微镜观察下表明，花背蟾蜍早期胚胎具有的两类表皮细胞在超微结构上的主要不同点是：无纤毛细胞含较多的线粒体、高尔基体和内质网等细胞以及大量的粘液泡，含较少的黑色素颗粒，细胞游离面具丰富的微突起。纤毛细胞含较多的黑色素颗粒、大量的卵黄小板和脂滴，很少能观察到细胞器，无粘液泡，细胞游离面具很多较长的纤毛，这些结构特点与它们执行不同的功能相关。