中国科技论文在线

上传时间

2009年03月17日

【期刊论文】Nickle(II) and cobalt(II) complexes of hydroxyl-substituted triazamacrocyclic ligand as potential antitumor agents

郑从义， Feng Liang， a Ping Wang， a Xiang Zhou， a Tao Li， b Zhaoyang Li， c Huakuan Lin， d， Dongzhao Gao， d Congyi Zhengc and Chengtai Wua， *

Bioorganic & Medicinal Chemistry Letters 14(2004)1901-1904，-0001，（）：

-1年11月30日

摘要

The stability constants for the formation of nickle(II) and cobalt(II) complexes of the ligand [1,4,7]triazecan-9-ol (L) were presented. Antitumor activity of two complexes was reported. Nuclei of [NiL]-stimulated BEL-7402 cells clearly exhibited condensation and break down into chromatin clumps typical of apoptosis. Also it exhibited perturbation effects to cell cycle, and optimal induction of apoptosis was found by Flow-Cytometric analysis. But CoL complex did not exhibit introduction effects to BEL-7402 cells apoptosis; and could not perturb cell cycle. NiL and CuL complexes could cleave supercoiled DNA (pBR 322 DNA) to nicked and linear DNA, and DNA of cells treated with NiL or CuL complex was obviously damaged; while CoL complex only could cleave supercoiled DNA (pBR 322 DNA) to nicked DNA, and DNA of cells treated with CoL complex had no significant difference with control.

Macrocyclic polyamines， Metal complexes， Antitumor activity， DNA cleavage.，

31浏览
0点赞
0收藏
0分享
63下载
0

引用

上传时间

2009年03月17日

【期刊论文】EXTREMELY LOW FREQUENCY (ELF) PULSED-GRADIENT MAGNETIC FIELDS INHIBIT MALIGNANT TUMOUR GROWTH AT DIFFERENT BIOLOGICAL LEVELS

郑从义， XINCHEN ZHANG， HUSHENG ZHANG*， CONGYI ZHENG， CHAOYANG LI， XINSONG ZHANG and WEI XIONG

Cell Biology International 2002, Vol. 26, No.7, 599-603，-0001，（）：

-1年11月30日

摘要

Extremely low frequency (ELF) pulsed-gradient magnetic field (with the maximum intensity of 0.6-2.0 T, gradient of 10-100T • M1, pulse width of 20-200 ms and frequency of 0.16-1.34Hz treatment of mice can inhibit murine malignant tumour growth, as seen from analyses at different hierarchical levels, from organism, organ, to tissue, and down to cell and macromolecules. Such magnetic fields induce apoptosis of cancer cells, and arrest neoangiogenesis, preventing a supply developing to the tumour. The growth of sarcomas might be amenable to such new method of treatment.

extremely low frequency (， ELF)， pulsed-gradient magnetic field， malignant tumour， inhibitory， biological levels.，

28浏览
0点赞
0收藏
0分享
57下载
0

引用

上传时间

2009年03月17日

【期刊论文】Microcalorimetric study of the metabolism of U-937 cells undergoing apoptosis induced by the combined treatment of hyperthermia and chemotherapy

郑从义， Liu Yuwena， Wang Cunxina， Zheng Congyib， *， Wu Haixiangb， Wang Zhiyonga， Qu Songshenga

Journal of Thermal Biology 27(2002)129-135，-0001，（）：

-1年11月30日

摘要

Hyperthermia is a useful adjunct in cancer therapy, as it can increase the effectiveness and decrease the toxicity of currently available cancer treatments such as chemotherapy and radiation. In this study we determined the power-time curves of U-937 cell line treated by the combination of hyperthermia and Carmofur by using an LKB 2277 Bioactivity Monitor. The maximal thermal power and the heat production were used to evaluate the antitumor effect. Our results show that the combined treatment of hyperthermia and Carmofur had a synergistic antitumor effect, which is consistent with the apoptosis ratio obtained by TUNEL assay. The results also indicate that the metabolic activity of apoptotic cells is lower than that of normal cells. Thus microcalorimetry is a powerful tool in fields of hyperthermia.

Microcalorimetry， Hyperthermia， Metabolism， Apoptosis， TUNEL

43浏览
0点赞
0收藏
0分享
50下载
0

引用

上传时间

2009年03月17日

【期刊论文】构建猪瘟病毒致细胞病变的PK-15细胞模型

郑从义，吴海祥，张楚瑜*，王家富，潘兹书，李蕾，曹晟，伊光辉

科学通报，2004，48（8）：822～826，-0001，（）：

-1年11月30日

摘要

猪瘟病毒在离体细胞培养条件下不引起宿主细胞病变，病毒与宿主细胞之间相互作用的研究一直没有合适的模型以猪瘟病毒中国标准强毒石门株为材料，通过基因工程的手段，在构建全基因组感染性克隆的基础上，对全基因组进行部分缺失，获得了7.5kh的亚基因组.以携带野生型猪瘟病毒的PK-15细胞为宿主，用亚基因组进行转染，获得到了能够诱导PK-15细胞产生CPE的亚基因组病毒，检测显示产生CPE的细胞培养物中野生型基因组和亚基因组共同存在猪瘟病毒致细胞病变模型的构建，为进一步研究其致病的分子机制开辟了新的方向。

猪瘟病毒，基因组感染性克隆，亚基因组，干扰缺损颗粒，致细胞病变效应

224浏览
0点赞
0收藏
0分享
45下载
0

引用

上传时间

2009年03月17日

【期刊论文】Following the rule: formation of the 6-helix bundle of the fusion core from severe acute respiratory syndrome coronavirus spike protein and identification of potent peptide inhibitors

郑从义， Jieqing Zhu， a， Gengfu Xiao， b， Yanhui Xu， c， Fang Yuan， d Congyi Zheng， b Yueyong Liu， a Huimin Yan， b David K. Cole， d John I. Bell， d Zihe Rao， c Po Tien， * and George F. Gaoa， d， *

Biochemical and Biophysical Research Communications 319(2004)283-288，-0001，（）：

-1年11月30日

摘要

Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) is a newly identified member of Family Coronaviridae. Coronavirus envelope spike protein S is a class I viral fusion protein which is characterized by the existence of two heptad repeat regions (HR1 and HR2) (forming a complex called fusion core). Here we report that by using in vitro bio-engineering techniques, SARS-CoV HR1 and HR2 bind to each other and form a typical 6-helix bundle. The HR2, either as a synthetic peptide or as a GSTfusion polypeptide, is a potent inhibitor of virus entry. The results do show that SARS-CoV follows the general fusion mechanism of class I viruses and this lays the ground for identification of virus fusion/entry inhibitors for this devastating emerging virus.

SARS-CoV， Coronavirus， Spike(， S)， protein， Fusion core， Heptad repeat (， HR1 and HR2)，， Inhibition

27浏览
0点赞
0收藏
0分享
22下载
0

引用