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张利平, Wen-Jun Li , Li-Ping Zhang , Ping Xu , Xiao-Long Cui , Zhi-Tang Lu , Li-Hua Xu and Cheng-Lin Jiang
International Journal of Systematic and Evolutionary Microbiology (2002), 52, 1695-1699,-0001,():
-1年11月30日
A psychrotolerant actinomycete, strain YIM6T, was isolated from a soil sample collected from Beijiang in Xinjiang Province, China. Both vegetative and aerial hyphae grew well on various media after 4 weeks. Long spore chains, borne on the aerial mycelium, were straight to flexuous or occasionally Retinaculiaperti and non-motile. The novel isolate grew well at between 8 and 20℃; the lower and upper temperature limits for growth were 0 and 32℃. The cell wall of strain YIM6T contained LL-diaminopimelic acid (A2pm) and traces of meso-A2pm. Whole-cell hydrolysates contained mainly glucose and small amounts of xylose, galactose and arabinose. The predominant menaquinones were MK9(H6) and MK-9(H8) and phosphatidylethanolamine was the diagnostic phospholipid. The predominant cellular fatty acids were 15∶0 anteiso, 16∶0 iso and 17∶0 cyclo. Phylogenetic analysis indicated that strain YIM6T belongs to the genus Streptomyces. In its morphology, physiological and biochemical characteristics and 16S rDNA sequence, strain YIM6T differed from other members of Streptomyces. Thus, strain YIM6T (=CCTCC 99005T=AS 4.1718T= DSM 41794T) is proposed as the type strain of a novel species, Streptomyces beijiangensis sp. nov.
Streptomyces beijiangensis sp., nov.,
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张利平, 石楠, 张利平*, 吕志堂, 杨润蕾, 刘志恒
微生物学报,2004,44(2):148~152,-0001,():
-1年11月30日
从云南地区土壤中分离到一株具有除莠活性的放线菌(Actinomycetes),对其产生的活性物质进行了分离、纯化及分析。从该菌株的发酵液中获得了有除莠活性的黄色结晶,薄板层析的结果表明,该结晶为多组分混和物,且每一组分均具有除莠活性。通过红外分析对其组分作了初步鉴定。同时对该菌株进行了形态、化学及系统发育学研究,根据16S rDNA 全序列构建了系统发育树,结果表明此菌株应属于链霉菌属(Streptomyces),同时具有耐低温和耐盐的特性。
链霉菌, 除莠活性, 活性产物, 系统发育
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【期刊论文】Streptosporangium subroseum sp. nov., an actinomycete with an unusual phospholipid pattern
张利平, Li-ping Zhang , Cheng-lin Jiang and Wen-xin Chen
International Journal of Systematic and Evolutionary Microbiology (2002), 52, 1235-1238,-0001,():
-1年11月30日
A strain of Streptosporangium with an unusual phospholipid pattern was isolated from Yunnan Province, a region in China. The isolate, designated CY-7113T, was identified by morphological and physiological properties, cell chemistry, genomic DNA GMC content, DNA–DNA hybridization and phylogenetic analysis. Based on 16S rRNA gene sequencing, DNA–DNA hybridization, phenotypic characteristics and its unusual phospholipid pattern, it was concluded that strain CY-7113T belongs to a novel Streptosporangium species, for which the name Streptosporangium subroseum sp. nov. is proposed. Strain CY-7113T (=CCTCC 97008T=CRC 16302T) is the type strain.
Streptosporangium subroseum sp., nov., ,, 16SrDNA,, actinomycete
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张利平, 刘淑君, 张俊强, 孙茜**, 刘志恒
微生物学报,38(3):233~236,1998,-0001,():
-1年11月30日
14 strains of Actinomycetes were isolated from soil samples collected in Yunnan, China Experiments showed that they had activity of herbicide. In this paper, the morphological and chemotaxonomic characters of these strains were studied and they were identified as three genera of Actinomycetales respecitively, such as Streptomyces. Nficromonospora and Nocardia.
放线菌,, 鉴定,, 除莠活性
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张利平, Wen-Jun Li , Ping Xu , Li-Ping Zhang, Shu-Kun Tang , Xiao-Long Cui , Pei-Hong Mao , Li-Hua Xu , Peter Schumann , Erko Stackebrandt and Cheng-Lin Jiang
International Journal of Systematic and Evolutionary Microbiology (2003), 53, 1421-1425,-0001,():
-1年11月30日
A halophilic actinomycete, strain YIM 90003T, was isolated from a soil sample collected from Xinjiang Province, China, by using starch-casein agar with a salt concentration of 20% (w/v), pH 7·0. The strain grew well on most media tested. No diffusible pigment was produced. Aerial mycelium and substrate mycelium were well developed on most media. The aerial mycelium formed short spore chains, bearing non-motile, straight to flexuous spores with wrinkled surfaces. The cell walls of strain YIM 90003T contained meso-diaminopimelic acid as the diagnostic diamino acid. Cell-wall hydrolysates contained galactose and arabinose. Menaquinone composition varied with the medium used for cell cultivation; on glucose-yeast extract medium supplemented with 10% NaCl, the major menaquinone was MK-9(H4), while, on vitamin-enriched ISP 2 medium, the major menaquinones were MK-10(H2), MK-9(H8) and MK-10(H4). Phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, diphosphatidyl glycerol, methylphosphatidylethanolamine, phosphatidylserine, phosphatidylcholine and an unidentified phospholipid. 16S rRNA gene sequence analysis showed Streptomonospora salina as the closest phylogenetic neighbour. On the basis of these analyses, strain YIM 90003T is a member of the genus Streptomonospora, though its properties do not match the generic description fully with respect to the menaquinone composition and peptidoglycan amino acid. Analyses of mechanically disrupted cell walls of the type species, Streptomonospora salina DSM 44593T, and strain YIM 90003T, purified by tryptic digestion and subsequent SDS treatment, revealed the exclusive presence of meso-diaminopimelic acid as the diagnostic diamino acid of peptidoglycan. Thus, the genus description of Streptomonospora, indicating the presence of several amino acids usually not found in the peptidoglycan moiety, is therefore emended. DNA–DNA hybridization and comparison of physiological and chemotaxonomic characteristics demonstrated strain YIM 90003T to be different from Streptomonospora salina. The name Streptomonospora alba sp. nov. is proposed, with strain YIM 90003T (=CCTCC AA001013T=DSM 44588T) as the type strain.
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