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【期刊论文】应用16SrRNA 基因V-2高变区序列进行链霉菌分子分类
张利平, 徐平, 余利岩
生物多样性,2001,9(2):129~137,-0001,():
-1年11月30日
用16SrRNA部分序列对Williams 数值分类系统所包含的链霉菌属种或种群进行系统进化分析。以包含16SrRNA 基因V-2高变区在内的120bp长核苷酸序列所作的系统进化树表明:这些链霉菌可分为34个簇,其中大簇5个(包括27~85株菌株);中等大小簇3个(包括9~12株菌);小簇8个(包括2~6株菌);单成员簇19个。结果同时表明,Williams 数值分类系统中根据形态、生理、生化特征进行归类而得的种或种群内存在较大异质性。
系统进化分析, 链霉菌, 16SrRNA基因
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张利平, Wen-Jun Li , Li-Ping Zhang , Ping Xu , Xiao-Long Cui , Zhi-Tang Lu , Li-Hua Xu and Cheng-Lin Jiang
International Journal of Systematic and Evolutionary Microbiology (2002), 52, 1695-1699,-0001,():
-1年11月30日
A psychrotolerant actinomycete, strain YIM6T, was isolated from a soil sample collected from Beijiang in Xinjiang Province, China. Both vegetative and aerial hyphae grew well on various media after 4 weeks. Long spore chains, borne on the aerial mycelium, were straight to flexuous or occasionally Retinaculiaperti and non-motile. The novel isolate grew well at between 8 and 20℃; the lower and upper temperature limits for growth were 0 and 32℃. The cell wall of strain YIM6T contained LL-diaminopimelic acid (A2pm) and traces of meso-A2pm. Whole-cell hydrolysates contained mainly glucose and small amounts of xylose, galactose and arabinose. The predominant menaquinones were MK9(H6) and MK-9(H8) and phosphatidylethanolamine was the diagnostic phospholipid. The predominant cellular fatty acids were 15∶0 anteiso, 16∶0 iso and 17∶0 cyclo. Phylogenetic analysis indicated that strain YIM6T belongs to the genus Streptomyces. In its morphology, physiological and biochemical characteristics and 16S rDNA sequence, strain YIM6T differed from other members of Streptomyces. Thus, strain YIM6T (=CCTCC 99005T=AS 4.1718T= DSM 41794T) is proposed as the type strain of a novel species, Streptomyces beijiangensis sp. nov.
Streptomyces beijiangensis sp., nov.,
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【期刊论文】Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae
张利平, Xiao-Long Cui , Pei-Hong Mao , Min Zeng , Wen-Jun Li , Li-Ping Zhang , Li-Hua Xu and Cheng-Lin Jiang
International Journal of Systematic and Evolutionary Microbiology (2001), 51, 357-363,-0001,():
-1年11月30日
Actinomycete strain YIM 90002T (=CCTCC 99003T=CCRC 16284T) was isolated from a soil sample collected from a salt lake in the west of China. The aerial mycelium of this organism is well developed but not fragmented and, at maturity, forms short chains of spores. Spores in short chains are oval-to rodshaped and have wrinkled surfaces. Substrate mycelium is branched with nonfragmenting hyphae and forms single oval to round spores borne on sporophores or dichotomously branching sporophores. Single spores have wrinkled surfaces. Single spores and spores in short chains are non-motile. Strain YIM 90002T contains meso-diaminopimelic acid, DD-diaminopimelic acid, glycine, lysine and aspartic acid in its cell wall and has glucose, galactose, ribose, xylose, arabinose and mannose as whole-cell sugars (no diagnostic sugars). The phospholipids are phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The major menaquinones are MK-9(H6), MK-10(H2) and MK-10(H4). Phylogenetic data indicate that this strain belongs to the family Nocardiopsaceae. The morphological and physiological characteristics and chemotaxonomic and phylogenetic data for this strain differ from those of previously described actinomycetes. Therefore, a new genus, Streptimonospora, is proposed for this organism; the type species of the genus is Streptimonospora salina gen. nov., sp. nov., and the type strain of S. salina is strain YIM 90002T.
Nocardiopsaceae,, Streptimonospora salina sp., nov., ,, 16S rDNA
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张利平, 石楠, 张利平*, 吕志堂, 杨润蕾, 刘志恒
微生物学报,2004,44(2):148~152,-0001,():
-1年11月30日
从云南地区土壤中分离到一株具有除莠活性的放线菌(Actinomycetes),对其产生的活性物质进行了分离、纯化及分析。从该菌株的发酵液中获得了有除莠活性的黄色结晶,薄板层析的结果表明,该结晶为多组分混和物,且每一组分均具有除莠活性。通过红外分析对其组分作了初步鉴定。同时对该菌株进行了形态、化学及系统发育学研究,根据16S rDNA 全序列构建了系统发育树,结果表明此菌株应属于链霉菌属(Streptomyces),同时具有耐低温和耐盐的特性。
链霉菌, 除莠活性, 活性产物, 系统发育
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【期刊论文】Streptosporangium subroseum sp. nov., an actinomycete with an unusual phospholipid pattern
张利平, Li-ping Zhang , Cheng-lin Jiang and Wen-xin Chen
International Journal of Systematic and Evolutionary Microbiology (2002), 52, 1235-1238,-0001,():
-1年11月30日
A strain of Streptosporangium with an unusual phospholipid pattern was isolated from Yunnan Province, a region in China. The isolate, designated CY-7113T, was identified by morphological and physiological properties, cell chemistry, genomic DNA GMC content, DNA–DNA hybridization and phylogenetic analysis. Based on 16S rRNA gene sequencing, DNA–DNA hybridization, phenotypic characteristics and its unusual phospholipid pattern, it was concluded that strain CY-7113T belongs to a novel Streptosporangium species, for which the name Streptosporangium subroseum sp. nov. is proposed. Strain CY-7113T (=CCTCC 97008T=CRC 16302T) is the type strain.
Streptosporangium subroseum sp., nov., ,, 16SrDNA,, actinomycete
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