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2009年07月07日

【期刊论文】Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae

张利平, Xiao-Long Cui , Pei-Hong Mao , Min Zeng , Wen-Jun Li , Li-Ping Zhang , Li-Hua Xu and Cheng-Lin Jiang

International Journal of Systematic and Evolutionary Microbiology (2001), 51, 357-363,-0001,():

-1年11月30日

摘要

Actinomycete strain YIM 90002T (=CCTCC 99003T=CCRC 16284T) was isolated from a soil sample collected from a salt lake in the west of China. The aerial mycelium of this organism is well developed but not fragmented and, at maturity, forms short chains of spores. Spores in short chains are oval-to rodshaped and have wrinkled surfaces. Substrate mycelium is branched with nonfragmenting hyphae and forms single oval to round spores borne on sporophores or dichotomously branching sporophores. Single spores have wrinkled surfaces. Single spores and spores in short chains are non-motile. Strain YIM 90002T contains meso-diaminopimelic acid, DD-diaminopimelic acid, glycine, lysine and aspartic acid in its cell wall and has glucose, galactose, ribose, xylose, arabinose and mannose as whole-cell sugars (no diagnostic sugars). The phospholipids are phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The major menaquinones are MK-9(H6), MK-10(H2) and MK-10(H4). Phylogenetic data indicate that this strain belongs to the family Nocardiopsaceae. The morphological and physiological characteristics and chemotaxonomic and phylogenetic data for this strain differ from those of previously described actinomycetes. Therefore, a new genus, Streptimonospora, is proposed for this organism; the type species of the genus is Streptimonospora salina gen. nov., sp. nov., and the type strain of S. salina is strain YIM 90002T.

Nocardiopsaceae,, Streptimonospora salina sp., nov., ,, 16S rDNA

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2009年07月07日

【期刊论文】Agromyces aurantiacus sp. nov., isolated from a Chinese primeval forest

张利平, Wen-Jun Li , Li-Ping Zhang , Ping Xu , Xiao-Long Cui , Li-Hua Xu , Zhongse Zhang , Peter Schumann , Erko Stackebrandt and Cheng-Lin Jiang

International Journal of Systematic and Evolutionary Microbiology (2003), 53, 303-307,-0001,():

-1年11月30日

摘要

A catalase-negative actinomycete, strain YIM 21741T, was isolated from a soil sample collected from a primeval forest at Xishuangbanna, Yunnan Province, China. Analysis of 16S rDNA showed the strain to be related to members of the genus Agromyces, with which it also shares morphological characteristics, e.g. branching hyphae breaking into diphtheroid and rod-like, irregular, non-motile fragments and a peptidoglycan containing the diagnostic amino acid 2,4-diamino-n-butyric acid. Whole-cell hydrolysates of strain YIM 21741T contained rhamnose and small quantities of glucose, galactose and mannose. The major menaquinone was MK-12, while MK-13 and MK-12 were minor components. Diagnostic phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the DNA was 72

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2009年07月07日

【期刊论文】粘细菌生态多样性的初步研究

张利平, 方晓梅, 张利平*

生物多样性,2001,9(3):207~213,-0001,():

-1年11月30日

摘要

通过对河北、云南、青藏高原不同地区的粘细菌进行生态多样性研究,从42 个样品中分离得到了10个属(A rchangium、Myxococcus、Cystobacter、Corallococcus、Melittangium、Sorangium、Polyangium、Chondromyces、Angiococcus、Stigmatella) 的150余株粘细菌,其中包括一些尚未有描述的菌株,有待鉴定。根据这些菌株的子实体结构、菌落形态、营养细胞、粘孢子形态等特征将它们初步鉴定到属。对这些特有自然生态环境的粘细菌按不同地点、植被、营养基质进行统计比较,结果表明粘细菌生态分布极为广泛,具有丰富的生态多样性。以上结果为粘细菌生物资源的有效开发利用奠定了基础。

粘细菌, 生态分布, 多样性

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2009年07月07日

【期刊论文】Improved methods of isolation and purification of myxobacteria and development of fruiting body formation of two strains

张利平, LiPing Zhang a, b, *, HaiYing Wang a, XiaoMei Fang a, Erko Stackebrandt c, YanBo Dinga b

Journal of Microbiological Methods 54(2003)21-27,-0001,():

-1年11月30日

摘要

By using baiting techniques and different purification methods, a high number of myxobacterial strains have been isolated as pure cultures from soil of different regions of China. Because myxobacterial cells do not disperse easily in liquid media, a medium containing an enzymatic hydrolysate of casein (CEH) medium have been used for purification and purity tests combined in a single step. The key method, in which isolates are reintroduced to sterile rabbit dung to induce fruiting bodies formation, facilitates purification of myxobacteria. Sterile rabbit dung pellets are used to mimic the natural growth substance of these organisms which has the advantage that characteristic fruiting bodies emerge, which is a key characteristics in the taxonomy of myxobacteria. In this study, the optimum program of isolation and purification of some myxobacteria strains has been established which will facilitate screening programs. Moreover, the development of fruiting body formation of strain BD20 (Chondromyces) and strain BD54 (Cystobacter) have been recorded in this study.

Myxobacteria, Isolation, Purification, Fruiting body

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2009年07月07日

【期刊论文】应用16SrRNA 基因V-2高变区序列进行链霉菌分子分类

张利平, 徐平, 余利岩

生物多样性,2001,9(2):129~137,-0001,():

-1年11月30日

摘要

用16SrRNA部分序列对Williams 数值分类系统所包含的链霉菌属种或种群进行系统进化分析。以包含16SrRNA 基因V-2高变区在内的120bp长核苷酸序列所作的系统进化树表明:这些链霉菌可分为34个簇,其中大簇5个(包括27~85株菌株);中等大小簇3个(包括9~12株菌);小簇8个(包括2~6株菌);单成员簇19个。结果同时表明,Williams 数值分类系统中根据形态、生理、生化特征进行归类而得的种或种群内存在较大异质性。

系统进化分析, 链霉菌, 16SrRNA基因

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    河北大学,河北

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