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张利平, Wen-Jun Li , Ping Xu , Li-Ping Zhang, Shu-Kun Tang , Xiao-Long Cui , Pei-Hong Mao , Li-Hua Xu , Peter Schumann , Erko Stackebrandt and Cheng-Lin Jiang
International Journal of Systematic and Evolutionary Microbiology (2003), 53, 1421-1425,-0001,():
-1年11月30日
A halophilic actinomycete, strain YIM 90003T, was isolated from a soil sample collected from Xinjiang Province, China, by using starch-casein agar with a salt concentration of 20% (w/v), pH 7·0. The strain grew well on most media tested. No diffusible pigment was produced. Aerial mycelium and substrate mycelium were well developed on most media. The aerial mycelium formed short spore chains, bearing non-motile, straight to flexuous spores with wrinkled surfaces. The cell walls of strain YIM 90003T contained meso-diaminopimelic acid as the diagnostic diamino acid. Cell-wall hydrolysates contained galactose and arabinose. Menaquinone composition varied with the medium used for cell cultivation; on glucose-yeast extract medium supplemented with 10% NaCl, the major menaquinone was MK-9(H4), while, on vitamin-enriched ISP 2 medium, the major menaquinones were MK-10(H2), MK-9(H8) and MK-10(H4). Phospholipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, diphosphatidyl glycerol, methylphosphatidylethanolamine, phosphatidylserine, phosphatidylcholine and an unidentified phospholipid. 16S rRNA gene sequence analysis showed Streptomonospora salina as the closest phylogenetic neighbour. On the basis of these analyses, strain YIM 90003T is a member of the genus Streptomonospora, though its properties do not match the generic description fully with respect to the menaquinone composition and peptidoglycan amino acid. Analyses of mechanically disrupted cell walls of the type species, Streptomonospora salina DSM 44593T, and strain YIM 90003T, purified by tryptic digestion and subsequent SDS treatment, revealed the exclusive presence of meso-diaminopimelic acid as the diagnostic diamino acid of peptidoglycan. Thus, the genus description of Streptomonospora, indicating the presence of several amino acids usually not found in the peptidoglycan moiety, is therefore emended. DNA–DNA hybridization and comparison of physiological and chemotaxonomic characteristics demonstrated strain YIM 90003T to be different from Streptomonospora salina. The name Streptomonospora alba sp. nov. is proposed, with strain YIM 90003T (=CCTCC AA001013T=DSM 44588T) as the type strain.
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张利平, 刘淑君, 张俊强, 孙茜**, 刘志恒
微生物学报,38(3):233~236,1998,-0001,():
-1年11月30日
14 strains of Actinomycetes were isolated from soil samples collected in Yunnan, China Experiments showed that they had activity of herbicide. In this paper, the morphological and chemotaxonomic characters of these strains were studied and they were identified as three genera of Actinomycetales respecitively, such as Streptomyces. Nficromonospora and Nocardia.
放线菌,, 鉴定,, 除莠活性
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【期刊论文】应用16SrRNA 基因V-2高变区序列进行链霉菌分子分类
张利平, 徐平, 余利岩
生物多样性,2001,9(2):129~137,-0001,():
-1年11月30日
用16SrRNA部分序列对Williams 数值分类系统所包含的链霉菌属种或种群进行系统进化分析。以包含16SrRNA 基因V-2高变区在内的120bp长核苷酸序列所作的系统进化树表明:这些链霉菌可分为34个簇,其中大簇5个(包括27~85株菌株);中等大小簇3个(包括9~12株菌);小簇8个(包括2~6株菌);单成员簇19个。结果同时表明,Williams 数值分类系统中根据形态、生理、生化特征进行归类而得的种或种群内存在较大异质性。
系统进化分析, 链霉菌, 16SrRNA基因
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张利平, 方晓梅, 张利平*
生物多样性,2001,9(3):207~213,-0001,():
-1年11月30日
通过对河北、云南、青藏高原不同地区的粘细菌进行生态多样性研究,从42 个样品中分离得到了10个属(A rchangium、Myxococcus、Cystobacter、Corallococcus、Melittangium、Sorangium、Polyangium、Chondromyces、Angiococcus、Stigmatella) 的150余株粘细菌,其中包括一些尚未有描述的菌株,有待鉴定。根据这些菌株的子实体结构、菌落形态、营养细胞、粘孢子形态等特征将它们初步鉴定到属。对这些特有自然生态环境的粘细菌按不同地点、植被、营养基质进行统计比较,结果表明粘细菌生态分布极为广泛,具有丰富的生态多样性。以上结果为粘细菌生物资源的有效开发利用奠定了基础。
粘细菌, 生态分布, 多样性
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张利平, 石楠, 张利平*, 吕志堂, 杨润蕾, 刘志恒
微生物学报,2004,44(2):148~152,-0001,():
-1年11月30日
从云南地区土壤中分离到一株具有除莠活性的放线菌(Actinomycetes),对其产生的活性物质进行了分离、纯化及分析。从该菌株的发酵液中获得了有除莠活性的黄色结晶,薄板层析的结果表明,该结晶为多组分混和物,且每一组分均具有除莠活性。通过红外分析对其组分作了初步鉴定。同时对该菌株进行了形态、化学及系统发育学研究,根据16S rDNA 全序列构建了系统发育树,结果表明此菌株应属于链霉菌属(Streptomyces),同时具有耐低温和耐盐的特性。
链霉菌, 除莠活性, 活性产物, 系统发育
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