含钙培养液（对照）和位用IAA处理的原生质体的体积和45Ca2+放射性强度均无变化。IAA处理含钙培养液中的原生质体。5min后45Ca2+积累明显增多，体积开始膨大。处理30min时45Ca2+积累最多，此时原生质体的膨大效应最好，随后45Ca2+积累和膨大效应逐渐下降。K+、2n2+、Ba2+、Mg2+等也可在一定程度上代替Ca2+使原生质体体积膨大。原生质体的吸水在膨大中起着一定作用。EGTA、LaCl3和verapamil均抑制IAA诱导的原生质体45Ca2+积累和体积膨大。说明Ca2+可能在6BA诱导原生质体嘭大的过程中起着重要作用。

This paper studied on the role of calcium in 6-BA-induced swelling of protoplasts isolated from hypocotyl in etiolated mung bean(Phaseolus radiatus L.) seedlig. Protoplasts incubated in CaCl2-bearing medium without hormone maintained a constant volume. To treat with 6-BA, they began to swell after 15 minutes, and continually swelled to the maximum volume 30 minutes later when they were treated with 10-1 mol/L 6-BA(Fig.2). However, the protoplasts could not swell when 6-BA was added into the medium without CaCl2(Fig.1). It was suggested that Ca2+ was necessary for 6-BA to induce protoplast swelling. 6-BA enabled the protoplasts to swell in less extent when K-. Zn2+, Ba2+or Mg2-instedad of Ca2-(Fig.3). Radioisotope experiments showed that K+influx incrased when Ca2+ as replaced by K+(Fig.4). Ca2+ accumulation in protoplasis treated by 6-BA was munch higher than that of control, and the time course of Ca2+ accumu-lation was similar to protoplasts swelling (Fig5). 45Ca2+level and the swelling of protoplasis were sharply declined when EGTA, verapamil or LaCcl3 was added into the medium(Table 1,2and3). These results indiated that Ca2+ may play an important role in 6-BA-induced swelling.

研究了超声技术促进蔗糖的酯化反应，考察了反应温度、时问、催化剂等对反应的影响。当蔗糖与辛酸乙酯的摩尔比为2∶1，反应温度为70℃，催化剂K2CO3占辛酸乙酯质量分数的11%和反应压力为11kPa的条件下，超声反应2h，能高产率地获得辛酸蔗糖酯，且产物的单酯化物含量达92%。产物结构用红外和核磁表征，产物易溶于极性溶剂，热稳定性好，亲水亲油平衡值（HLB）为10.8。

蓝光，白光和黑暗叶绿豆下胚轴愈伤组织形成和生长过程中蛋白质代谢的影响不同。培养后3-18d，蓝光处理材料的可溶性蛋白质含量明显高于白光处理，更高于黑暗培养的材料。蓝光和白光明显促进3H-亮氨酸掺入蛋白质，而蓝光和白光处理后游离氨基酸含量与黑暗对照相比，下降时间早，幅度太。在培养过程中，蛋白酶活性的变化与游离氨基酸相似。蛋白质合成抑制荆环己酰亚胺（CHM）抑制愈伤组织生长，其中以蓝光最大，白光次之，黑暗最小。在培养基中加入CHM愈早，抑制程度愈大。实验表明，CHM抑制愈伤组织蛋白质合成，也是以蓝光最甚。由此可见，蓝光促进绿豆下胚轴愈伤组织的形成、生长和蛋白质合成，低蛋白质含量，同时抑制细胞脱分化。此外，在绿豆子叶愈伤组织形成过程中，代表蛋白质合成能力的多聚核糖体相对量在子叶切段离体培养的第5天和第7天增加到原来的3倍多，表明蛋白质合成能力迅速增强（谭保才等1993）。然而，这些组织培养研究均未涉及光质的影响。本实验以不同光质处理绿豆下胚轴切段，研究愈伤组织形成和生长过程中蛋白质含量、蛋白质酶活性及蛋白质合成的动态变化，以此了解蓝光影响其蛋白质代谢的特征。

本文采用单克隆抗体酶联免疫吸附分析法测定了UV-B诱导DNA产生的CPD和6-4PP。经0.5mW/cm2UV-B处理15min的小牛胸腺和鲱鱼精DNA，CPD和6-4PP含量显著增加，而未经UV-B处理的对照DNA则没有二聚体形成。