从土样中分离筛选出产β-甘露聚糖酶的地衣芽孢杆菌，经紫外线诱变处理后（15W，30cm照射10s）获得高酶活力NK-27菌珠，在以魔芋粉、豆饼粉为碳、氮原添加无机盐的发酵培养基中，β-甘露聚糖酶活力达110.49μ/ml。初始pH值、装液量、培养温度和培养时间对产酶有一定影响。

纯化后的链霉菌S01菌株几丁质酶用环柱法在PDA平板上对杨树腐烂病菌（Valsa sordida）、葡萄孢菌（Botrytis sp. AS3.2616）、黄瓜黑腥病菌（Cladosporium cucumerinum）、辣椒疫病菌（Phytophthora capsici）、棉花黄萎病菌（Verticillium albo-atrum）、立枯丝核菌（Rhizoctonia solani）等植物病原真菌及产黄青霉（Penicillum chrysogenum）、啤酒酵母（Saccharomyces cerevisiae）的生长具有明显的抑制作用。在6株植物病原真菌的液体培养物中加入几丁质酶后，菌丝出现扭曲变形、细胞质聚集、外溢等异常现象。在高渗缓冲液中，经几丁质酶处理后，啤酒酵母、葡萄孢菌和产黄青霉的细胞壁受到破坏，释放出了原生质体。

由地衣芽孢杆菌NK-27获得的β-甘露聚糖酶在40℃、酶液终浓度1u/ml时，经12h水解魔芋粉、解豆胶、瓜儿豆胶和田菁胶所生成的酶解产物，经薄层层析检测表明为单糖和低聚糖。在PYG和GAM液体培养基中，添加不同量的四种植物胶酶解产物对青春双歧杆菌（Bifidobacterium adolescentis）具有明显的促生长作用，菌体增殖数从107提高到108个/ml。

A new engineering strain, Bacillus pumilus c172-14 (pBX 96), was obtained by introducing the pBX 96 plasmid, which carries the α-amylase amy gene, into the host strain of alkalophilic Bacillus pumilus c172 via transformation. The newly constructed strain was found to express the amy gene and could use starch instead of glucose or starch hydrolysate as carbon source for its fermentation of alkaline protease. The pBX 96 plasmid in the new host was found to be segregationally and structurally stable. The expression of amy gene did not affect the host strain's resistance to bacteriophages. Moreover, the level of alkaline protease was improved significantly compared with the parent strain. The constructed strain gave a maximum alkaline protease activity of 14, 014U/ml in shaking flask after 48h cultivation when growing in a medium containing 6% corn meal, 4% soybean flour, 0.4% Na2HPO4, 0.03% KH2PO4, 0.02% MgCl2, 0.3% CaCl2, 0.25% Na2CO3, 0.1% glucose, and 20μg/ml kanamycin (pH 7.0). The optimal pH value and temperature of the alkaline protease were 11.0 and 40℃, respectively. This enzyme was stable over a pH range of 8-11. Its residual activity remained at 100% when treated under a temperature of less than 45℃ for 30min. The corresponding residual activity reduced to 65% of its optimal value at 60℃ for 30min. The alkaline protease was a kind of serine protease, which was demonstrated by the complete inactivation by PMSF (1mM). This newly constructed strain will be useful in the alkaline protease industry.

地衣芽孢杆菌（Bacillus licheniformis）NK-27菌株发酵产生的β-甘露聚糖酶（β-mannanase）经硫酸铵盐析沉淀，两次DEAE纤维素和Sephadex G-100离子交换柱层析以及制备PAGE等步骤，获得了凝胶电泳均一的样品。用SDS-凝胶电泳测得纯化后的β-甘露聚糖酶分子量为26kD，用凝胶聚焦电泳测得等电点PI为5.0。酶反应的最适pH为9.0，最适温度为60℃，稳定pH为6.0-9.0，稳定温度为40℃。金属离子中Mg2+、Ca2+、Fe2+、Fe2+、Ni2+对该酶有一定的激活作用；而Sn2+、Zn2+、Al3+、Ag+和Hg2+对该酶有强烈的抑制作用。NK-27菌株的β-甘露聚糖酶对魔芋葡萄甘露聚糖和角豆胶半乳甘露聚糖的Km值分别为7.14和5.56mg·ml-1；Vmax分别为200.53和157.45μmol·mg-1·min-1。