[1] OU S H. Rice disease. 2nd ed.[M]. Surrey: Commonwealth Mycology Institute, 1985. [2] ROSSMAN A Y, HOWARD R J, VALENT B. Pyricularia grisea, the correct name for the rice blast disease fungus[J]. Mycologia, 1990, 84: 509-512. [3] HAMER J E, HOWARD R J, CHUMLEY F G, et al. A mechanism for surface attachment in spores of plant pathogenic fungus[J]. Science, 1988, 239: 288-290. [4] HOWARD R J. Cell biology of pathogenesis[A]. ZEIGLER R S, LEONG S A, TENG P S. Rice Blast Disease[M]. Wallingford, UK: CAB International, 1995. 3-22. [5] TALBOT N J. Having a blast: exploring the pathogenicity of Magnaporthe grisea[J]. Trends in Microbiology, 1995, 3: 9-16. [6] WEINTRAUB R T, MILLER W E, SCHANTZ E J. Chemical stimulation of germination of spores of Piricularia oryzae[J]. Phytopathology, 1958, 48: 7-10. [7] BOURETT T M, PICOLLELLI M A, HOWARD R J. Postembedment labeling of intracellular concanavalin A-binding sites in freeze-substituted fungal cells[J]. Experimental Mycology, 1993, 17: 223-235. [8] 林福呈，李德葆. 植物病原真菌附着胞形成及其基因表达调节[J]. 植物病理学报，1997, 27 (3): 193-197 [9] BOURETT T M. HOWARD R J. Ultrastructural immunolocation of actin in a plant pathogenic fungus[J]. Protoplasma, 1991. 163: 199-202. [10] DE JONG J C, McCORMACK B J, SMIRNOFF N, et al. Glycerol generates turgor in rice blast[J]. Nature, 1997, 389: 244-245. [11] MONEY N P. Osmotic pressure of aqueous polyethylene glycols[J]. Plant Physiology, 1989, 91: 766-769. [12] BOURETT T M, HOWARD R J. Actin in penetration pegs of the fungal rice blast pathogen, Magnaporthe grisea[J]. Protoplasma, 1992, 168: 20-26. [13] HOWARD R J, FERRARI M A. Role of melanin in appressorium function[J]. Experimental Mycology, 1989, 13: 403-418. [14] MONEY N P. Measurement of pore size in the hyphal cell wall of Achlya bisexualis[J]. Experimental Mycology, 1990, 14: 234-242. [15] KUBO Y, FURUSAWA I. Melanin biosynthesis. Prerequisite for successful invasion of the fungal plant host by appressoria of Colletotrichum and Pricularia[A]. COLE G T, HOCH H C. THE Fungal Spore and Disease Initiation in Plants and Animals[M] New York, USA: Plenum Press, 1991. 205-218. [16] HOWARD R J, FERRARI M A, ROACH D H, et al. Penetration of hard substrates by a fungus employing enormous turgor pressures[J]. Proc. Natl. Acad. Sci. USA, 1991, 88: 11281-11284. [17] KOLAITUKUDY P E. Enzymatic penetration of the plant cuticle by fungal pathogens[J]. Ann. Rev. Phytopathol., 1985, 23: 223-250. [18] SWEIGARD J A, CHUMLEY F G, VALENT B. Disruption of a Magnaporthe grisea cutinase gene[J]. Molecular and General Genetics, 1992, 232: 183-190. [19] BECHINGER C, GIEBEL K F, SCHNEL M. Optical measurements of invasive forces exerted by appressoria of a plant pathogenic fungus[J]. Science, 1999, 286: 1896-1899

Eleven high effective monoclonal antibodies to Magnaporthe grisea were screened out from 230 hybrid cell strains showing positive reaction in ELISA tests, among which monoclonal antibody 2B4 showed more highly banding to cell wall surfaces of conidia, germ tubes, appressoria, germ tubes, appressoria or/and second infective hypha by indirect-immunofluorcscence test. Ultrastructural analysis and the antigen localisition of monocloual antibody 2B4 with immuno-gold labeling found this antigen protein exists extensively on cell wails of the diftbrrent life stages above, and is a secretion protein. 6 positive cDNAs, MP1, MP2, MP3, MP4, MP5 and MP6 were screened out with 2B4 from conidium cDNA expression library. A 31kDa protein expressed from a positive clone MP1 in a prokaryotic in vitro translation system was consistent with a 15kda protein from the extractive of the fungal surfaces, and its gene cloning and functional analysis are undertaking.

A total of 985 bacterial isolates were obtained from rice leaves and other grasses. The isolates were cultared in nutrient broth (NB) medium. After Centrifugated, 8000r/min for 10min, the supernatants of each bacterial NB cultures were tested for their suppressive effects on the germination and appressorium formation of Magnaporthe grisea. Of the tested isolates, 13 isolates showed suppressive effects. Three isolates identified as Psendomonas fluorescens (Xh216), Bacillus sp. (Xh222) and Pseudomonas. Sp. (Xh240), suppressed the disease severities on the detached rice leaves with the disease severity reduction 72.3%, 70.5% and 67.4%, respectively.