中国科技论文在线

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2009年07月07日

【期刊论文】Separation of New Antidiabetic Agent, N-(trans-4-isopropylcyclohexylca r bonyl)-D-phenylalanine and three Related Compounds by RP-HPLC

杨更亮， Gengliang Yang ，， * Haiyan Liu ， Haiying Li ， Yan huan Wang ， Zhiwei Li ， Yi Chen

Chromatographia 57(2003)245-247，-0001，（）：

-1年11月30日

摘要

Column liquid chromatography， Antidiabetic agent， N-(， trans,， cis-4-isopropylcyclohexylcarbonyl)， -D-phenylalanine， Trans,， cis-4-isopropylcyclohexanecarboxyhc acid

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2009年07月07日

【期刊论文】Monitoring of the conversion from triptolide to tripchlorolide in triptergium wilfordii by micellar electrokinetic caillary chromatography

杨更亮， Baozhi Li a， Jie Bai c， Gengliang Yang a， b， *， Zhiwei Li a， Lijuan Wang a， Yi Chen b

Journal of Chromatography A 1097(2005)199-202，-0001，（）：

-1年11月30日

摘要

A novel concocting method to convert Triptolide (T) into Tripchlorolide (T4) in the traditional Chinese herb Tripterygium wilfordii Hook F. and a micellar electrokinetic capillary chromatographic (MEKC) approach by which the conversion of Triptolide (T) and Tripchlorolide(T4) was identified and determined had been established. Investigations of the influence of different pH values of boric acid and borax buffer and of sodium dodecyl sulfate (SDS) and organic additive concentrations had been carried out, and the optimum separation for T and T4 was achieved using boric acid and borax of pH 7.0 with 30mM SDS and 20% (volume ratio) methanol as the running buffer. It was found that MEKC exhibited good accuracy, precision and repeatability and the content of T4 was greatly increased in the herb that was treated by the new concocting method.

Micellar Electrokinetic capillary chromatography， Triptolide， Tripchlorolide， Tripterygium wilfordii Hook F

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2009年07月07日

【期刊论文】Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique

杨更亮， Hongyuan Yan a， b， Gengliang Yang a， ∗， Fengxia Qiao b， Yi Chen a

Journal of Pharmaceutical and Biomedical Analysis 36(2004)169-174，-0001，（）：

-1年11月30日

摘要

A micellar electrokinetic chromatography (MEKC) method was developed for the determination of nateglinide in animal plasma by on-line sweeping technique, in which plasma samples were simply deproteinized with acetonitrile, and analyzed with 16 mmol/L NaH2PO4+6mmol/L Na2B4O7+60mmol/L sodium dodecyl sulfate (SDS) (pH 7.14) as the running buffer, a fused-silica capillary as the separation tube, 21 kV as the running voltage and UV detection at 214nm. Under these conditions, more than 100-fold enrichment of nateglinide was obtained with the good linear relation in the range of nateglinide plasma concentration 0.2-7mg/L (R=0.998). The method could be applied successfully to determine trace drugs in clinical analysis.

MEKC， On-line sweeping， Nateglinide， Plasma

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2009年07月07日

【期刊论文】Chiral separation of Tamsulosin isomers by HPLC using cellulose Tris(3,5-dimethhylphenylcarbamate) as a chiral stationary phase

杨更亮， Zhefeng Zhang a， Gengliang Yang a， c， ∗， Guijian Liang b， Haiyan Liu a， Yi Chen c

Journal of Pharmaceutical and Biomedical Analysis 34(2004)689-693，-0001，（）：

-1年11月30日

摘要

A high-performance liquid chromatographic (HPLC) method was developed for the chiral separation of an antagonist of alpha1A adrenoceptors, tamsulosin and its S-isomer. Baseline separation of the isomers was achieved within 35 min on a CHIRALCEL OD-RH column with a binary solvent mixture of 50 mmol l−1 KPF6-acetonitrile (v/v (70∶30), pH 5.0) as the optimized mobile phase. The detection limits and quantification limits of both R-isomer and S-isomer were 0.11 and 0.44 ng,respectively. The R.S.D. values of peak-area for the two isomer were 0.42% (of peak-height∶0.77%) for R-isomer and 0.64%(of peak-height∶0.92%) for S-isomer (n=5).

Chiral separation， Tamsulosin (， 5-[(， 2R)， -2-{， [2-(， 2-ethoxyphenoxy)， ethyl]amino}， -propyl]-2-methoxy-benzenesulfonamide)，， Isomer， Cellulose tris(， 3,， 5-dimethylphenylcarbamate)， stationary phase

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2009年07月07日

【期刊论文】Chiral Separation of Nateglinide and its L Enantiomer on a Molecularly Imprinted Polymer-Based Stationary Phase

杨更亮， Gengliang Yang ，， Junfa Yin ， Zhiwei Li ， Haiyan Liu ， Liping Cai ， Dexian Wang ， Yi Chen

Chromatographia 59(2004)705-708，-0001，（）：

-1年11月30日

摘要

A new chiral stationary phase for nateglinide (N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine) based on a molecularly imprinted polymer has been prepared by non-covalent imprinting. For chromatographic analysis the effects on the separation of mobile phase composition, flow rate, and temperature were investigated, and the optimum conditions for HPLC were shown to be: mobile phase, acetonitrile; flow rate, 0.5mL min-1; temperature, 25℃. It was shown that the nateglinide-imprinted polymer was capable of recognizing the enantiomeric difference between nateglinide and its L enantiomer, whereas the non-imprinted polymer had no such ability. Scatchard analysis was used to investigate the binding characteristics of the nateglinide-imprinted stationary phase; this indicated that two classes of binding site were present in the imprinted polymer. The equilibrium dissociation constant (KD) and the apparent maximum number (Qmax) of high-and low-affinity binding sites were 3.7×10-4mol L-1 and 11.38µmol g-1, and 1.81×10-3mol L-1 and 27.73µmol g-1,respectively.

Column liquid chromatography， Molecularly imprinted stationary phase， Chiral separation， Nateglinide

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