中国科技论文在线

上传时间

2009年07月07日

【期刊论文】Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique

杨更亮， Hongyuan Yan a， b， Gengliang Yang a， ∗， Fengxia Qiao b， Yi Chen a

Journal of Pharmaceutical and Biomedical Analysis 36(2004)169-174，-0001，（）：

-1年11月30日

摘要

A micellar electrokinetic chromatography (MEKC) method was developed for the determination of nateglinide in animal plasma by on-line sweeping technique, in which plasma samples were simply deproteinized with acetonitrile, and analyzed with 16 mmol/L NaH2PO4+6mmol/L Na2B4O7+60mmol/L sodium dodecyl sulfate (SDS) (pH 7.14) as the running buffer, a fused-silica capillary as the separation tube, 21 kV as the running voltage and UV detection at 214nm. Under these conditions, more than 100-fold enrichment of nateglinide was obtained with the good linear relation in the range of nateglinide plasma concentration 0.2-7mg/L (R=0.998). The method could be applied successfully to determine trace drugs in clinical analysis.

MEKC， On-line sweeping， Nateglinide， Plasma

39浏览
0点赞
0收藏
0分享
159下载
0

引用

上传时间

2009年07月07日

【期刊论文】Separation of New Antidiabetic Agent, N-(trans-4-isopropylcyclohexylca r bonyl)-D-phenylalanine and three Related Compounds by RP-HPLC

杨更亮， Gengliang Yang ，， * Haiyan Liu ， Haiying Li ， Yan huan Wang ， Zhiwei Li ， Yi Chen

Chromatographia 57(2003)245-247，-0001，（）：

-1年11月30日

摘要

Column liquid chromatography， Antidiabetic agent， N-(， trans,， cis-4-isopropylcyclohexylcarbonyl)， -D-phenylalanine， Trans,， cis-4-isopropylcyclohexanecarboxyhc acid

34浏览
0点赞
0收藏
0分享
71下载
0

引用

上传时间

2009年07月07日

【期刊论文】Monitoring of the conversion from triptolide to tripchlorolide in triptergium wilfordii by micellar electrokinetic caillary chromatography

杨更亮， Baozhi Li a， Jie Bai c， Gengliang Yang a， b， *， Zhiwei Li a， Lijuan Wang a， Yi Chen b

Journal of Chromatography A 1097(2005)199-202，-0001，（）：

-1年11月30日

摘要

A novel concocting method to convert Triptolide (T) into Tripchlorolide (T4) in the traditional Chinese herb Tripterygium wilfordii Hook F. and a micellar electrokinetic capillary chromatographic (MEKC) approach by which the conversion of Triptolide (T) and Tripchlorolide(T4) was identified and determined had been established. Investigations of the influence of different pH values of boric acid and borax buffer and of sodium dodecyl sulfate (SDS) and organic additive concentrations had been carried out, and the optimum separation for T and T4 was achieved using boric acid and borax of pH 7.0 with 30mM SDS and 20% (volume ratio) methanol as the running buffer. It was found that MEKC exhibited good accuracy, precision and repeatability and the content of T4 was greatly increased in the herb that was treated by the new concocting method.

Micellar Electrokinetic capillary chromatography， Triptolide， Tripchlorolide， Tripterygium wilfordii Hook F

24浏览
0点赞
0收藏
0分享
56下载
0

引用

上传时间

2009年07月07日

【期刊论文】硅胶整体柱的制备及对苯取代物的分离

杨更亮，高文惠，，杨更亮*，杨静，刘海燕，尹俊发，陈义

分析化学研究报告，2004，32（3）：295～298，-0001，（）：

-1年11月30日

摘要

研究了一种制备硅胶整体柱的新方法。该工艺以四甲氧基硅烷和聚乙二醇为原料，采用改进的溶胶-凝胶技术，建立了消除柱体开裂和变形弯曲的方法及中孔的构建方法，考察了流速对柱压降的影响，并在正相色谱模式下应用制备的硅胶整体柱对苯取代化合物进行了分离。结果表明采用新工艺所制备的硅胶整体柱不开裂，不变形，并具有通孔和中孔双孔结构，渗透性强，柱压降低，机械强度高，柱效高等特点。这些特性尤其适合化合物的高效快速分离。

硅胶整体柱，溶胶-凝胶技术，孔结构，正相色谱

36浏览
0点赞
0收藏
0分享
38下载
0

引用

上传时间

2009年07月07日

【期刊论文】Chiral Separation of Nateglinide and its L Enantiomer on a Molecularly Imprinted Polymer-Based Stationary Phase

杨更亮， Gengliang Yang ，， Junfa Yin ， Zhiwei Li ， Haiyan Liu ， Liping Cai ， Dexian Wang ， Yi Chen

Chromatographia 59(2004)705-708，-0001，（）：

-1年11月30日

摘要

A new chiral stationary phase for nateglinide (N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine) based on a molecularly imprinted polymer has been prepared by non-covalent imprinting. For chromatographic analysis the effects on the separation of mobile phase composition, flow rate, and temperature were investigated, and the optimum conditions for HPLC were shown to be: mobile phase, acetonitrile; flow rate, 0.5mL min-1; temperature, 25℃. It was shown that the nateglinide-imprinted polymer was capable of recognizing the enantiomeric difference between nateglinide and its L enantiomer, whereas the non-imprinted polymer had no such ability. Scatchard analysis was used to investigate the binding characteristics of the nateglinide-imprinted stationary phase; this indicated that two classes of binding site were present in the imprinted polymer. The equilibrium dissociation constant (KD) and the apparent maximum number (Qmax) of high-and low-affinity binding sites were 3.7×10-4mol L-1 and 11.38µmol g-1, and 1.81×10-3mol L-1 and 27.73µmol g-1,respectively.

Column liquid chromatography， Molecularly imprinted stationary phase， Chiral separation， Nateglinide

24浏览
0点赞
0收藏
0分享
109下载
0

引用