中国科技论文在线

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2009年07月07日

【期刊论文】Chiral separation of Tamsulosin isomers by HPLC using cellulose Tris(3,5-dimethhylphenylcarbamate) as a chiral stationary phase

杨更亮， Zhefeng Zhang a， Gengliang Yang a， c， ∗， Guijian Liang b， Haiyan Liu a， Yi Chen c

Journal of Pharmaceutical and Biomedical Analysis 34(2004)689-693，-0001，（）：

-1年11月30日

摘要

A high-performance liquid chromatographic (HPLC) method was developed for the chiral separation of an antagonist of alpha1A adrenoceptors, tamsulosin and its S-isomer. Baseline separation of the isomers was achieved within 35 min on a CHIRALCEL OD-RH column with a binary solvent mixture of 50 mmol l−1 KPF6-acetonitrile (v/v (70∶30), pH 5.0) as the optimized mobile phase. The detection limits and quantification limits of both R-isomer and S-isomer were 0.11 and 0.44 ng,respectively. The R.S.D. values of peak-area for the two isomer were 0.42% (of peak-height∶0.77%) for R-isomer and 0.64%(of peak-height∶0.92%) for S-isomer (n=5).

Chiral separation， Tamsulosin (， 5-[(， 2R)， -2-{， [2-(， 2-ethoxyphenoxy)， ethyl]amino}， -propyl]-2-methoxy-benzenesulfonamide)，， Isomer， Cellulose tris(， 3,， 5-dimethylphenylcarbamate)， stationary phase

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2009年07月07日

【期刊论文】Determination of nateglinide in animal plasma by micellar electrokinetic chromatography and on-line sweeping technique

杨更亮， Hongyuan Yan a， b， Gengliang Yang a， ∗， Fengxia Qiao b， Yi Chen a

Journal of Pharmaceutical and Biomedical Analysis 36(2004)169-174，-0001，（）：

-1年11月30日

摘要

A micellar electrokinetic chromatography (MEKC) method was developed for the determination of nateglinide in animal plasma by on-line sweeping technique, in which plasma samples were simply deproteinized with acetonitrile, and analyzed with 16 mmol/L NaH2PO4+6mmol/L Na2B4O7+60mmol/L sodium dodecyl sulfate (SDS) (pH 7.14) as the running buffer, a fused-silica capillary as the separation tube, 21 kV as the running voltage and UV detection at 214nm. Under these conditions, more than 100-fold enrichment of nateglinide was obtained with the good linear relation in the range of nateglinide plasma concentration 0.2-7mg/L (R=0.998). The method could be applied successfully to determine trace drugs in clinical analysis.

MEKC， On-line sweeping， Nateglinide， Plasma

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2009年07月07日

【期刊论文】Adsorption Isotherms on Aminoantipyrine Imprinted Polymer Stationary Phase

杨更亮， G.Yang ，， *， D.Wang ， Z.Li ， S.Zhou ， Y.Chen

Chromatographia 58(2003)53-58，-0001，（）：

-1年11月30日

摘要

Column liquid chromatography， Molecular imprinted polymers， Adsorption isotherms

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2009年07月07日

【期刊论文】原位聚合那格列奈分子印迹手性固定相的分子识别特性研究

杨更亮，尹俊发a， b，杨更亮*， a，张轶华b，刘海燕b，陈义a.*

化学学报，2004，62（19）：1922～1926，-0001，（）：

-1年11月30日

摘要

以手性药物那格列奈为模板分子，采用原位聚合法制备了具有特定识别性能和手性拆分能力的分子印迹聚合物，并用作高效液相色谱固定相实现了那格列奈与其对映体的手性拆分。通过静态结合方法考察了该聚合物的选择结合能力，并讨论了手性分离过程中的热力学性质。结果表明，原位聚合法制备的棒状聚合物固定相对模板分子及其对映体有很好的手性拆分性能。

分子印迹聚合物，手性固定相，原位聚合，手性拆分，那格列奈

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2009年07月07日

【期刊论文】Chiral Separation of Nateglinide and its L Enantiomer on a Molecularly Imprinted Polymer-Based Stationary Phase

杨更亮， Gengliang Yang ，， Junfa Yin ， Zhiwei Li ， Haiyan Liu ， Liping Cai ， Dexian Wang ， Yi Chen

Chromatographia 59(2004)705-708，-0001，（）：

-1年11月30日

摘要

A new chiral stationary phase for nateglinide (N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine) based on a molecularly imprinted polymer has been prepared by non-covalent imprinting. For chromatographic analysis the effects on the separation of mobile phase composition, flow rate, and temperature were investigated, and the optimum conditions for HPLC were shown to be: mobile phase, acetonitrile; flow rate, 0.5mL min-1; temperature, 25℃. It was shown that the nateglinide-imprinted polymer was capable of recognizing the enantiomeric difference between nateglinide and its L enantiomer, whereas the non-imprinted polymer had no such ability. Scatchard analysis was used to investigate the binding characteristics of the nateglinide-imprinted stationary phase; this indicated that two classes of binding site were present in the imprinted polymer. The equilibrium dissociation constant (KD) and the apparent maximum number (Qmax) of high-and low-affinity binding sites were 3.7×10-4mol L-1 and 11.38µmol g-1, and 1.81×10-3mol L-1 and 27.73µmol g-1,respectively.

Column liquid chromatography， Molecularly imprinted stationary phase， Chiral separation， Nateglinide

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