The effects of oligosaccharide from Fusarium oxysprum on secondary metabolisms in suspension cultures of Taxus chinensis var. mairei were investigated by detecting the oxidative burst (OB) signals using an Electron Paramagnetic Resonance (ESR) spectrometer. The oxidative burst signals induced by oligosaccharide gradually increased to a maximum at 4h. Phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) activities were respectively 2 and 4 times higher than those of control, and large amount of phenolics were accumulated in culture medium. Two times of obvious increase in taxol production was observed, which was in accordance with the two times of elicitation of oxidative burst signals. The dependence of taxol production on the intensity of OB signals obeyed a modified Logistic curve. It was inferred that the syntheses of the side chain and nucleus of taxol were enhanced at low and high intensities of OB signals, respectively.

运用顺磁共振、电镜、能谱等手段研究了寡聚糖对红豆杉细胞氧化还原生。理态势的影响。结果表明，诱导前红豆杉细胞处于相对稳定的生长态势，细胞完整，细胞器发达；氧化还原电势较低，活性氧相关酶系统相对稳定, 细胞初生代谢旺盛，紫杉醇合成速率很低。诱导后红豆杉细胞向产物合成态势转移，SOD酶活性迅速提高，CAT和POD酶活被强烈抑制；细胞出现活性氧迸发，4h达最大值；细胞的氧化还原电势增高，细胞膜上离子通道被激活，H+和Ca2+ 内流，Cl-外流；液胞内出现大量含Ca2+离子的高电子致密区并呈规律性变化，培养环境碱化；细胞生长被抑制，紫杉醇合成速率升高，其产量提高了515倍，达7611mg·L-1。

利用热带假丝酵母（Candida tropicalis）突变株SP-UV-56进行十三碳二元酸发酵，研究了pH值控制模式对产酸的影响。低pH值对维持菌体活性有利，但不利于菌体产酸；高pH值能促进菌体产酸，但菌体活性有所下降。在研究中定义了3个参数pHe值、Cp，e和ΔpHe。菌体的产酸与ΔpHe有关，通过实验建立了pHe值与产酸量Cp,e之间的关联式，提出了pH值梯度控制策略，并确定了适宜的ΔpHe。以ΔpHe=012为推动力，建立了pH值连续控制方程。应用pH值连续控制方程进行发酵，转化120h产酸量达到145.2g·L-1，较对照组的产酸量提高了16%，适宜的pH值控制范围为715～812，为长链二元酸发酵技术实现pH值连续自动控制奠定了理论基础。

利用Klebsiella pneumoniae生长与催化耦合的特点，将好氧生长与厌氧转化两个过程耦合，开发了两段双底物发酵生产1,3-丙二醇的新工艺，并对其工艺特点进行了初步研究。结果表明，采用两段双底物发酵工艺，发酵过程菌体浓度明显高于传统的厌氧转化工艺，OD650最大值达到9.37，发酵60h,1,3-丙二醇的浓度达到50.16g/L，生成速率达0.836g/(L×h)，比传统工艺提高了45%。

综述了近年来工业生物催化过程的发展，提出按照生物催化剂的制备和催化过程的特点将工业生物催化分成生长耦联型催化和生长非耦联型催化，通过这两类生物催化成功的范例以及研究进展讨论生物催化工业化的途径和方法及进一步发展的方向和对策。